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County of San Luis Obispo
Public Health Laboratory
Ambient water testing

Ambient (recreational and ocean) water testing


Total Coliforms and E. coli MPN, undiluted (Order code: 8350)

Method

Enzyme substrate—Chromogenic                                                

Standard Methods 9223 B-2016 Colilert

Description

This test employs the IDEXX, Inc Colilert™ Quantitray system, allowing the detection and enumeration of total coliforms by hydrolysis of ortho-nitrophenyl-ß-D-galactoside and Escherichia coli by hydrolysis of 4-methyl-umbelliferyl-ß-D-glucuronide. Enumeration results in the reporting of a Most Probable Number (MPN) and is commonly used to determine the microbial load of both the total coliforms and E. coli. 

Reference range

Results for total coliforms and E. coli results range from <1 MPN to >2419.6 MPN for 100 mL of undiluted sample. Recommended limits vary by water source.

Turnaround time

1 day


Total Coliforms and E. coli MPN, 1:10 dilution (Order code: 8025)

Method

Enzyme substrate—Chromogenic                                               

Standard Methods 9223 B-2016 Colilert

Description

This test employs the IDEXX, Inc Colilert™ Quantitray system, allowing the detection and enumeration of total coliforms by hydrolysis of ortho-nitrophenyl-ß-D-galactoside and Escherichia coli by hydrolysis of 4-methyl-umbelliferyl-ß-D-glucuronide. Enumeration results in the reporting of a Most Probable Number (MPN) and is commonly used to determine the microbial load of both the total coliforms and E. coli. This is the most commonly used test for determination of safety of recreational water. 

Reference range

For marine waters used for recreation, total coliforms should be less than 10,000 MPN per 100 mL for a single sample and an average of less than 1,000 MPN for a 30-day sampling period; E. coli should be less than 400 MPN per 100 mL for a single sample and an average of less than 200 MPN for a 30-day sampling period.

Turnaround time

1 day

 

Enterococci MPN, undiluted (Order code: 8110)

Method

Defined substrate hydrolysis—Chromogenic                      

Standard Methods 9230 D-2013 Enterolert

Description

This test employs the IDEXX, Inc Enterolert™ Quantitray system, allowing the detection and enumeration of Enterococci by hydrolysis of 4-methyl-umbelliferyl glucoside. Enumeration results in the reporting of a Most Probable Number (MPN) and is commonly used to determine the microbial load of enterococci, an enteric bacterium that is easily and accurately detected and enumerated in marine and brackish waters.  

Reference range

Enterococci MPN should be less than 104 MPN per 100mL for a single sample and an average of less than 35 MPN for a 30-day sampling period for recreational waters to be considered safe for recreational activities, such as swimming.

Turnaround time

1 day


Enterococci MPN, 1:10 dilution (Order code: 8010)

Method

Defined substrate hydrolysis—Chromogenic                     

Standard Methods 9230 D-2013 Enterolert

Description

This test employs the IDEXX, Inc Enterolert™ Quantitray system, allowing the detection and enumeration of Enterococci by hydrolysis of 4-methyl-umbelliferyl glucoside. Enumeration results in the reporting of a Most Probable Number (MPN) and is commonly used to determine the microbial load of enterococci, an enteric bacterium that is easily and accurately detected and enumerated in marine and brackish waters.  

Reference range

Enterococci MPN should be less than 104 MPN per 100mL for single sample and less than 35 MPN for a 30-day sampling period for recreational waters to be considered safe for recreational activities, such as bathing.

Turnaround time

1 day

County of San Luis Obispo Public Health Laboratory
2191 Johnson Avenue, San Luis Obispo 93401
www.slocounty.gov/PH-Lab