MALDI-TOF MS yeast identification

TEST DESCRIPTION

Identification of yeast isolates using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS). MALDI-TOF MS technology analyzes the proteins, mainly ribosomal proteins, of a microorganism. The instrument converts the microorganism’s proteins into a protein profile or fingerprint. This protein profile is compared to profiles of microorganisms in a reference database, and the identity of a yeast is confirmed when a close match is found to a reference profile.

Methodology

MALDI-TOF mass spectrometry

TEST FREQUENCY & RESULT AVAILABILITY

Performed weekly. Results are typically available one week after the yeast has been isolated.

ACCEPTABLE SPECIMEN TYPES AND COLLECTION

A yeast isolate submitted on solid media (preferably slant culture)
Patient specimens submitted for fungal culture will be eligible and reflexed for testing once a yeast has been isolated from the specimen

SPECIMEN STORAGE

For yeast isolates submitted on solid media, store and transport at room temperature (17–25 °C/63–77 °F). All culture containers should be taped securely and double bagged when submitted.

SPECIMEN TRANSPORT

Shipping conditions should follow the temperature and time requirements outlined above.

REJECTION CRITERIA

Yeast isolates that are non-viable
Yeast isolates that arrive in culture containers that are damaged during transit or are not properly labeled with patient identifiers

CODES

CPT: 87106
LOINC: 98403-9
Test code: 3950

TEST FEE

Refer to the current fee schedule.