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County of San Luis Obispo
Public Health Laboratory
Mycology ID procedure

Mycology (fungal) identification procedure

TEST DESCRIPTION

Determination of fungal genus and/or species using microscopy and other methods. Microscopic examination is performed using lactophenol cotton blue staining for visualization of fungal structures. Additional methods may include slide culture, growth studies, and sporulation induction.   

Methodology

Fungal identification methods

 

TEST FREQUENCY & RESULT AVAILABILITY

Performed daily to weekly. Identification may require ≥2 weeks after initial detection/isolation of a mold.

 

ACCEPTABLE SPECIMEN TYPES AND COLLECTION

  • A mold isolate submitted on solid media (preferably slant culture)
  • Patient specimens submitted for fungal culture will be eligible once a mold has been isolated from the specimen

 

SPECIMEN STORAGE

For mold isolates submitted on solid media, store and transport at room temperature (17–25 °C/63–77 °F). All culture containers should be taped securely and double bagged when submitted. 

Note: If Coccidioides immitis is suspected, Category A (UN2814) packaging is required. Please contact the laboratory if Category A shipping supplies are needed.

 

SPECIMEN TRANSPORT

Shipping conditions should follow the temperature and time requirements outlined above.

 

REJECTION CRITERIA

  • Mold isolates that are non-viable
  • Mold isolates that arrive in culture containers that are damaged during transit or are not properly labeled with patient identifiers

 

CODES

CPT: 87107
LOINC: 42805-2
Test code: 3980

 

TEST FEE

Refer to the current fee schedule.

County of San Luis Obispo Public Health Laboratory
2191 Johnson Avenue, San Luis Obispo 93401
www.slocounty.gov/PH-Lab